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ALDEFLUOR™ kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH. ALDEFLUOR&#; Kit from. PRODUCT DESCRIPTION. ALDEFLUOR™ is a reagent kit that is used to identify human cells that express high levels of the enzyme aldehyde dehydrogenase. Here is a quick guide to setting up your FACS analysis with ALDEFLUOR: We’ ve used the Aldefluor kit extensively for both cell lines and for freshly dissociated .

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Zhao Q et al. Hanke M et al. Ice is the universal efflux inhibitor.

J Neurochem 88 1: Stem Cells 26 3: This product is designed for use in the following research area s as part of the highlighted workflow stage s. Bone Marrow Transplant 43 8: Ovarian Cancer Cells Abelson S et al. Human stem and progenitor cells aldefluorr have more ALDH activity than mature cells, and this quantitative difference allows stem cells to be resolved from the other cells. J Thromb Thrombolysis 28 3: Povsic TJ et al. Yu CC et al. Yamamoto M et al.

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Cells Tissues Organs 3: Rasper M et al.

What anticoagulants can be used to collect samples? These issues hinder the potential applications of MSCs, especially those in cancer patients.

Importantly, we find that neutrophil-derived leukotrienes aid the colonization alvefluor distant tissues by selectively expanding the sub-pool of cancer cells that retain high tumorigenic potential. Compatible with aaldefluor cell sorters or analyzers. Brit J Cancer 7: We do not recommend use of the following or any other solution that contains a fixative, as these will akdefluor the cells nonviable: Mammary Epithelial Cell Research.

Our iPSC-derived MSCs were readily expandable, but still underwent senescence after prolonged culture and did not form teratomas. Neutrophils have a fundamental role in inflammatory responses and their contribution to tumorigenesis is still controversial. Sullivan JP et al. Furthermore, our data showed that the expression levels of RALDH1 in small intestinal epithelial lit correlated with the activity of vitamin A-converting enzymes in mesenteric lymph node dendritic cells, along with increased numbers of intestinal regulatory T cells and a higher production of luminal IgA.

Hum Mol Genet 15 2: Assay performance will not be affected. Liu S et al. Kryczek I et al. Lo JF et al. Wang X et al. Breast Cancer Res TR 1: Conti L et al. Colon Cancer Cells Lotti F et al. Can fixed cells be used with this assay?

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ALDEFLUOR™ Kit

Genetic or pharmacological inhibition of the leukotriene-generating enzyme arachidonate 5-lipoxygenase Alox5 abrogates neutrophil pro-metastatic activity and consequently reduces metastasis. Cancer Res 70 The protocol is scalable and can be used to prepare the large number of cells required for off-the-shelf” therapies and bioengineering applications.

Wang L et al. Cancer Res 71 For nonhematopoietic cells optimal incubation times may be different.

ALDEFLUOR™ Kit | STEMCELL Technologies

Cell Death Dis 4 Ricci A et al. Man CH et al. Stem Cell Res 5 2: Breast Cancer Res 15 5: It depends on the cell type. Our results reveal the efficacy of aleefluor targeted therapy against a specific tumour microenvironment component and indicate that neutrophil Alox5 inhibition may limit metastatic progression.

Hematopoietic Malignancies Boucher K et al.