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DETERMINACIÓN DE ACTIVIDAD ENZIMÁTICA DE LA FOSFATASA ÁCIDA DE GERMEN DE TRIGO Y PROPIEDADES CINÉTICAS DE LA FOSFATASA. Método Hillmann: La fosfatasa ácida a pH hidroliza el – naftilfosfato o fosfato Fosfatasa acida Prostática (U/L). = x (∆E/min Transcript of FOSFATASA ÁCIDA. FOSFATASA ÁCIDA Es una enzima Isoenzima Prostática o isoenzima 5 – Importante en la detección de.

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Prostatic acid phosphatase in serum and semen of dogs. The incidence of prostatic malignancy has increased the use of tissue markers to detect cancer. Tissue specific antigens or differentiation antigens are found on the surface of normal cells. Clinically, these antigens are important to diagnose alterations in the tissues and for immunotherapy.

The objective of the present study was to evaluate the prostatic acid phosphatase concentration in blood and seminal plasma of intact and healthy dogs at different ages. The evaluation was carried out by spectrophotometer, using a commercial kit.

The prostatic acid phosphatase PAP levels did not differ according to the age and did not correlate with age or prostatic dimensions verified by ultrasonography.

The PAP concentration values varied greatly within each group. However, more studies are necessary to evaluate the role of prostatic acid phosphatase in the canine prostate and fosfztasa importance as a diagnostic test for prostate disorders. Although the prostate is found in all mammals, it has greater importance in men, dogs and chimpanzees Steiner et al due to the frequency of disorders.

Archivos de medicina veterinaria – Fosfatasa ácida prostática en suero y semen de perros

The dog has been used as a model to study the development of prostate disease in human beings, because it is the one of the animal species that spontaneously develops prostatic hyperplasia and prostatic adenocarcinoma Barsanti and Finco Prostatic adenocarcinoma is the main prostatic neoplasia in humans and dogs, especially in middle aged and elderly individuals and medium to large sized dogs Swinney Some authors have suggested that the great majority of cases occur in dogs older prosstatica 8 years of age average 8.

The risk of developing cancer is 2.

The high prevalence of prostatic malignity has increased the use of tissue markers to detect cancer Alivizatos et al Tissue specific antigens are found on the surface of normal cells. Clinically, these antigens are important to diagnose alterations in the tissues and for immunotherapy Souza and Toniollo Markers that reflect the biological activity of the tumor support the decision cosfatasa how aggressive a treatment should be Lewenhaupt et al In humans, the detection of increased levels of prostatic glucoproteins in serum, mainly prostate specific antigen PSA and prostatic acid phosphatase PAPis important for early diagnosis.

Prostatic acid phosphatase can be found in high concentrations in the serum and prostatic tissue of healthy men and in higher concentra tions in the serum of patients with disseminated prostatic cancer. Therefore, it had been used for years as a diagnostic test for prostatic cancer in men until fosgatasa discovery of PSA Proostatica et al However, for veterinary patients, tissue markers of cancer are not routinely used for screening purposes in Brazil.

In Europe, some laboratories have developed a test to detect canine prostate specific esterase CPSE in canine serum to diagnose prostate diseases such as BPH or prostatitis. Phosphatase is one of the most abundant prostatic proteins of the human seminal fluid, which is the best source for isolation and purification.

However, prostatic acid phosphatase is also found in most human tissue, such as liver, kidneys, spleen, placenta, erythrocytes, brain, leucocytes, blood serum and others Fink et alOstrowski and Kuciel It could be a restrictive factor for using PAP as a diagnostic tool, because serum concentrations are high in benign prostatic hyperplasia BPH and in non-prostatic diseases Alivizatos et al However, the highest expression of PAP occurs in the prostate Solin et al and its increase in fosfagasa is probably due to a degeneration of the prostatic secretory epithelial cells induced by prostatic alterations.

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The use of PAP measurement in human serum is important for therapeutic monitoring and prognostic evaluation Lewenhaupt et alGraddis et al As in humans, the PAP secretion in dogs is hormone-dependent, but the quantitative alterations are less evident in fosfatasaa prostate of these animals because acixa concentration of this prostaticaa is lower than that from the human fosaftasa. Immunohistochemical methods and immunoeletronic microscopy have demonstrated that the enzyme is a high sensitive marker of the structural integrity of the prostate secretor epithelium in dogs McEntee et alSouza and Toniollo Some authors Corazza et al have demonstrated promising results on the use of PAP as a biochemical marker adida the differentiation between prostatic adenocarcinoma and BPH.

FOSFATASA ÁCIDA by Andrés Santos Santamaria on Prezi

The results obtained showed that, in normal males, the PAP concentration in serum increased significantly with age. Dogs with adenocarcinoma presented higher PAP levels than those with BPH, normal dogs and dogs with non-prostatic diseases. Another author Souza found age differences for PAP values, suggesting that it would decrease according to the age.

Nevertheless, other authors Bell et al found that PAP serum and seminal activity did not differ between normal dogs and dogs with prostatic disease, nor there was not any difference in PAP concentrations in serum and seminal fluid among dogs with varied prostatic disease. The objective of this study was to quantify the PAP concentration in serum and seminal fluid fosfaasa healthy dogs and verify correlation between PAP levels and age or prostatic dimensions.

Thirty-six intact, mixed breed, male dogs, prostaticx clinical signs of reproductive or prostatic diseases, were divided into three groups G of twelve dogs, according to age: The body weight of the animals varied from 6.

All animals were examined clinically before any procedure. Anamnesis, physical exam and palpation of the prostate to assess its size, consistency, symmetry and location were carried out.

The dogs were examined by ul-trasonography 1with sectorial 5. After physical examination, a blood sample was obtained from the cephalic vein, labeled and sent to the laboratory to be centrifuged at g, during five minutes, to obtain the serum. The semen was collected by manual stimulation, using a sterilized funnel and collector tube. The first and second fractions were disposed of and the third one was collected because it is richer in prostatic fluid and has fewer cells.

The prostatic fluid was placed in a glass tube and taken to the flsfatasa and centrifuged at 1. The serum and seminal plasma were usually collected in the morning. This determination uses monophosphate timolftaleine as substrate because it is highly specific for the prostatic isoenzyme.

The concentrations were evaluated by spectrophotometer 3.

For statistical analysis the averages and standards deviations of PAP serum and seminal concentrations were obtained and also the prostate dimensions from each group. The Shapiro-Wilk normality test was applied to all the parameters evaluated, as recommended for studies with less than 50 observations. All of them met the normality requirements kurtosis and skewnessexcept the semen PAP concentrations that were not skewness.

The Bartlett test showed the homogeneity prostafica the data. The Pearson correlation test was performed to evaluate the relation among the parameters evaluated. The semen PAP concentration data was submitted to radicial transformation.

Analysis of variance for ventral dorsal and craniocaudal prostate dimension, prostatic acid phosphatase PAP concentrations in serum and semen of dogs. The prostate shape, size, contour and integrity were ;rostatica determined by ultrasonography. The canine prostate was predominantly round with a regular surface and presented homogenous parenchyma fosratasa, characterized by hyperechoic areas within lower echogenicity areas transonic regions in all animals of GI and GII, but little anechoic sites were observed fosvatasa only one dog of GIII, suggesting micro cysts related to BPH.

The ultrasonographic exam was essential to characterize the morphology of the canine prostate and detect probable cavity lesions.

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The round form was reported previously Cartee and RowlesCooney et alBussadori Prostate homogeneity texture was reported Bus sadoriMatoon and Nylandbut it is still a controversy. Muzzi verified an increased diffuse parenchyma echogenicity in altered prostate; but, in the present study, no alteration was observed in echogenic pattern even in cases of increased prostate or micro cysts.

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Prostate dimensions were close to previous reports Ruel fosfataea al prrostatica, Di Santis et al in dogs at same ages. Statistical analysis verified that there were no significant differences in serum or semen PAP concentrations among the groups Table 2.

However, the PAP concentrations varied within each group, presenting values from 0. There was no correlation prostatcia serum and semen PAP concentration, nor there protatica any correlation between levels of serum or semen PAP and age or prostatic dimensions. Another study Souza noticed lower PAP levels in the serum when the age increased. However, it is similar to Reiman results that found no correlation between serum PAP concentrations and age or body weight.

Amorim et al evaluated PAP concentrations in serum and urine of middle age years old and old dogs years old and observed difference only in urinary concentrations between the two groups. The values of this enzyme pre sented a high coefficient of variation in the present study, which prosatica high heterogeneity of the individual values, maybe due to a sub prostatkca non-prostatic disease.

It could suggest that the substrate used to measure the PAP concentration was not very specific. The PAP values in the serum were lower than those obtained in some other studies Prostatlca et alSouzaAmorim et alMoura et al This could be explained by the difference in the reagent used by those fosfataas, which contained tartarate, a PAP inhibitor, and the reagent used in this study, phosphate timolftaleine, a substrate for PAP. The concentrations of this enzyme were higher in the prostatkca plasma than in the serum, confirming its prostatic origin, as described previously Rosecrans et al The measurement of this enzyme in the prostatic fluid is important, because variations due to alterations in the gland can be easily detected, since this material has more PAP.

Presence of PAP was observed in prostativa urine Amorim et alMoura et albut the levels were lower than those observed for seminal plasma in that study. There was a variation in the serum and seminal PAP values within each studied in the present study, endorsing a previous report in which values of PAP found in the serum of 90 human patients with BPH ranging from 0. Hence, it may be suggested that maximum and minimum values for this enzyme range within large intervals.

Although the prostatic acid phosphatase concentrations did not vary in relation to age, we might not reject it as an important tissue marker.

Thus, others studies are necessary to evaluate the prosfatica of prostatic acid phosphatase measurement as fosgatasa diagnostic test for prostate disease. Efficacy of eight serially measured markers for diagnosis of prostatic carcinoma. Arch Esp Urol 45, Arq Bras Med Vet Zootec 56, Cytochemistry and biochemistry of acid phosphatases VII: Clinical and pathologic features of prostatic adeno-carcinoma in sexually intact and castrated dogs: J Am Vet Med Assoc Evaluation of serum and seminal plasma markers in the diagnosis of canine prostate disorders.

J Vet Intern Med 9, Ecografia della prostata nel cane. Praxis Vet 14, Cartee RE, T Rowles. Transabdominal protatica evaluation of the prostatcia prostate. Vet Radiol 24, Ultrasonography of the canine prostate with histologic correlation. Serum total prostatic and non-prostatic acid phosphatase in healthy dogs and in dogs with prostatic diseases. J Small Anim Pract 35, Tissue kallikrein of human seminal plasma is secreted by the prostate gland.

Biol Chem Hoppe-Seyler Prostatic acid phosphatase expression in human tissues. Int J Clin Exp Pathol 4,